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Image Search Results


Journal: Cell Stem Cell

Article Title: Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells

doi: 10.1016/j.stem.2018.10.008

Figure Lengend Snippet:

Article Snippet: For transduction, human CB-derived hematopoietic stem/progenitor cells (HSPC) were cultured in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin (100 IU/ml), streptomycin (100 μg/ml), 100 ng/ml recombinant human stem cell factor (rhSCF), 20 ng/ml recombinant human thrombopoietin (rhTPO), 100 ng/ml recombinant human Flt3 ligand (rhFlt3), and 20 ng/ml recombinant human IL6 (rhIL6) (all from Peprotech) 16 to 24 hours prior to transduction.

Techniques: Blocking Assay, Purification, Control, Recombinant, Cell Isolation, Isolation, shRNA, Software, Plasmid Preparation, Real-time Polymerase Chain Reaction